| date | | Thursday, September 16th 2010, 17 c.t. |
| location | | G2-104, CeBiTec Building |
| speaker | | Prof. Dr. Johannes WienbergChrombios GmbH and Department Biology II, University of Munich, Germany |
| title | | Flow cytometry and molecular cytogenetics as tools for the analysis of genome evolution
|
| | | Sorting of chromosomes by fluorescence activated cell sorting (FACS) and molecular cytogenetics (fluorescence in situ hybridization, FISH) have made comparative genome mapping applications more pertinent than ever before. FACS can provide the molecular probes for FISH (chromosome painting) and for DNA sequencing approaches for any mammalian species. Recent comparative chromosome painting results demonstrate that the gross organization of the genome of Eutheria (placental mammals) into chromosomes follows a simple basic architecture that is almost completely conserved for more than 100 million years in various species of almost all extant mammalian orders. Only some few minor changes can be observed when comparing the human and the proposed ancestral eutherian karyotype. The "default" frequency of gross rearrangements would calculate less than two changes within 10 million years of mammalian evolution. The main changes are translocations, fissions, and fusions of large chromosome segments or of chromosome arms. Chromosome numbers may have changed significantly up and down in this fusion/fission process, but in most instances the main architecture still remains evident. Knowledge of this basic organization of mammalian chromosome architecture can assist in the assembly of DNA sequence data in new sequencing programs. |
| host | | Prof. Dr. Alfred Pühler |
