| date | | Monday, May 2nd 2011, 15 c.t. |
| location | | G2-104, CeBiTec Building |
| speaker | | Dr. Klaus MayerInstitute of Bioinformatics and Systems Biology
Helmholtz Zentrum München
German Research Center for Environmental Health
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| title | | A poor man's approach to unlock cereal genomes
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| | | Barley is an important food and feed crop. It is closely related to wheat and its 5.2 gigabase genome is archaetypical for the Triticeae. Genome complexity and a repeat content of >80% are major obstacles to unlock the genome by systematic sequencing. We use a powerful approach of chromosome sorting, low pass shotgun sequencing, gene-array hybridisation and bioinformatic integration of model grass genome synteny information to obtain detailed insight into the organization of the barley genome. The gene content of barley is estimated to comprise ~32,000 genes and roughly 22,000 genes were positioned along the barley genome. Detailed comparisons to rice, sorghum and Brachypodium reveals vast differences in local conservation with regions that likely have undergone rearrangements and deletions in barley, rice, sorghum or Brachypodium. The resolution also allows to undertake genome scale comparisons to wheat and to analyse for genes that are under selection in the respective species. We present a highly ordered and information-enriched scaffold of the barley genome that reaches – at a fraction of the costs – a level of information density and resolution for a representative diploid Triticeae genome.
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| host | | Prof. Dr. Bernd Weisshaar |
