CeBiTec – Colloquium
Monday, May 20, 2019, 17 c.t.
G2-104, CeBiTec Building
Dr. Nayelli Marsch-Martinez
Laboratory of Plant Cell Identity
Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV) Irapuato (Mexico)
Regulatory networks of pistil and fruit development in Arabidopsis thaliana
The loss of WIP2 or NO TRANSMITTING TRACT (NTT) function leads to developmental defects in pistils and roots. WIP2 is a zinc finger transcription factor, and when it is overexpressed it can alter the development of plant organs. Moreover, the triple mutant of WIP2 and two closely related genes leads to a very severe phenotype, highlighting an essential role for this kind of transcription factors in specific tissues. Knowing genes that act downstream of WIP2 can be very useful to understand more about its role in regulating developmental programs. Therefore, we are exploring downstream candidate genes. One was previously identified using strategies such as yeast-one-hybrid assays. WIP2 presents interactions with other proteins, and other targets were recently identified by analyzing the co-expression networks of WIP2 and one of its interactors (SEEDSTICK, STK). This led also to the description of new altered phenotypes of one of their common targets. In order to find more genes acting downstream, some of which may be related to hormonal pathways, we have recently performed RNA-seq analysis using a line where NTT activity can be induced. We are currently analyzing the data obtained.
Host: Prof. Dr. Bernd Weisshaar