Jasmonic acid (JA) and its biosynthetic precursor 12-oxo-phytodienoic acid (OPDA) are well known as mediators of plant development and stress responses. In tomato, the JA-insensitive mutant jai1 exhibits defects in female develop­ment leading to female sterility but shows additionally an early senescence phenotype of stamens. To identify putative JA-dependent regulatory compo­nents in stamen and ovule development, isolated gametophytic organs from wild type and jai1 were used for a comparative transcriptomic approach. Two genes encoding MYB transcription factors (SlMYB21 and SlMYB24) were among the high number of differentially regulated genes and were subjected to further functional charac­terization. Regarding plant’s wound response, jasmonates have been described as main mediators. Here, OPDA was described to have distinct signaling properties in Arabidopsis. To investigate OPDA signaling, the wound-induced transcriptomes of JA- and OPDA-deficient mutants were compared to that of the wild type. Endogenous OPDA showed no unique transcriptional signature upon wounding, whereas application of OPDA triggered a distinct response suggesting compartmentalization of endoge­nously formed OPDA. Trans-organellar comple­mentation was used to clarify the possible compartmentation of OPDA and revealed that OPDA might primarily serve as a JA precursor with limited independent signaling functions in the early wound response of Arabidopsis.